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dc.contributor.advisorBrauer, Philipen_US
dc.contributor.authorGorny, Kristie Nicoleen_US
dc.date.accessioned2014-06-17T13:29:53Z
dc.date.issued1997en_US
dc.identifier.urihttp://hdl.handle.net/10504/55679
dc.description.abstractStudies show that urokinase-type plasminogen activator (uPA) conversion of plasminogen into plasmin is an important mediator of cell migration. In the developing chick heart, uPA has been implicated as a mediator of atrioventricular (AV) cushion cell migration, however the role of the plasminogen/plasmin system has not been examined. The purpose of this study was to test the hypothesis that uPA activation of plasminogen to plasmin increases cardiac mesenchymal cell migration in vitro. The first part of the study assessed stage 17/18 chicken AV explant lysates for their ability to convert plasminogen into plasmin. They were shown to activate chicken plasminogen and produce plasmin. The lysates did not have any detectable tissue-type plasminogen activator. Next, the AV explants were cultured on 3-dimensional collagen gels and the migratory capacity of cardiac mesenchymal cells was assessed in the presence or absence of various test reagents. Addition of human and chicken plasminogen, or aprotinin (an inhibitor of plasmin) had no effect on cell migration. However, an anticatalytic-chicken uPA antibody, but not non- immune IgG, significantly decreased cell migration at all concentrations tested. These results showed that (1) AV segments can activate plasminogen and that an anticatalytic uPA IgG can block this activity, (2) the plasminogen/plasmin system is not required for cardiac mesenchymal cell migration in vitro, and (3) uPA mediates cardiac mesenchymal cell migration in vitro independent of its ability to convert plasminogen into plasmin.Therefore, these results reject the hypothesis that uPA activation of plasminogen to plasmin increases cardiac mesenchymal cell migration in vitro.en_US
dc.language.isoen_USen_US
dc.publisherCreighton Universityen_US
dc.rightsCopyright is retained by the Author. A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above.en_US
dc.subject.meshCell Movementen_US
dc.subject.meshHeart--physiologyen_US
dc.subject.meshPlasminogen Activatorsen_US
dc.subject.meshPlasminogenen_US
dc.titleRole of Plasminogen and Urokinase-Type Plasminogen Activator in Cardiac Mesenchymal Cell Migration In Vitroen_US
dc.typeThesis
dc.rights.holderKristie Nicole Gornyen_US
dc.publisher.locationOmaha, Nebraskaen_US
dc.description.noteProQuest Traditional Publishing Optionen_US
dc.description.pagesvii, 58 leavesen_US
dc.contributor.cuauthorGorny, Kristie Nicoleen_US
dc.degree.levelMS (Master of Science)en_US
dc.degree.disciplineBiomedical Sciences (graduate program)en_US
dc.degree.nameM.S. in Biomedical Sciencesen_US
dc.degree.grantorGraduate Schoolen_US
dc.degree.committeeYee, Johnen_US
dc.degree.committeePatterson, Ericen_US
dc.degree.committeeVollberg, Thomasen_US


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