Show simple item record

dc.contributor.advisorCullen, Diane M.en_US
dc.contributor.authorTanner, Sharon J.en_US
dc.date.accessioned2015-05-05T14:32:01Z
dc.date.available2015-06-20T13:40:20Z
dc.date.issued2002en_US
dc.identifier.urihttp://hdl.handle.net/10504/68513
dc.description.abstractBone is an organ that is highly sensitive to changes in its mechanical loading environment. Although it is known that increased mechanical loading results in new bone formation, the cellular mechanisms underlying this response remain unclear. This investigation applied in vivo and in vitro methodologies to examine the responses of three stages of the osteoblast lineage (osteogenic colony forming unit (CFU-O), pre-osteoblast, and osteoblast) to short-term, external mechanical loads applied to the tibiae of rats. The results showed that loading protocols sufficient to induce lamellar bone formation do not affect endocortical CFU-O number or the alkaline phosphatase activity, collagen content, calcium content, or total cell number of the osteoblasts within each colony. Loading protocols sufficient to induce woven bone formation stimulated periosteal preosteoblasts to proliferate within 36 h after load application, and contribute new osteoblasts to the adaptive response within 48 h. Finally, apoptosis was suppressed during the early (1-5 d) post-load period, apparently to maintain the number of osteoblasts and pre-osteoblasts contributing to ongoing load-induced osteogenesis. These results, combined with previous examinations of post-load cellular kinetics (Turner, 1998a), indicate that the adaptive response to short-term loading involves the proliferation of pre-osteoblasts leading to the addition of new osteoblasts to the active forming surface. The recruitment of CFU-Os may be dependent on strain magnitude (which in turn determines the type of bone formed) or on the duration of loading (short-term vs. long term).en_US
dc.language.isoen_USen_US
dc.publisherCreighton Universityen_US
dc.rightsCopyright is retained by the Author. A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above.en_US
dc.subject.meshOsteoblastsen_US
dc.subject.meshRatsen_US
dc.subject.meshStress, Mechanicalen_US
dc.titleCellular Responses to Short-Term Mechanical Loading: Relative Involvement of Osteoblasts, Pre-Osteoblasts, and Osteoprogenitor Cellsen_US
dc.typeDissertation
dc.rights.holderSharon J. Tanneren_US
dc.publisher.locationOmaha, Nebraskaen_US
dc.description.noteProQuest Traditional Publishing Optionen_US
dc.description.pagesv, 130 pagesen_US
dc.contributor.cuauthorTanner, Sharon J.en_US
dc.degree.levelPhD (Doctor of Philosophy)en_US
dc.degree.disciplineBiomedical Sciences (graduate program)en_US
dc.degree.namePh.D. in Biomedical Sciencesen_US
dc.degree.grantorGraduate Schoolen_US
dc.degree.committeeYee, John A.en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record